Affinity Cryo-Electron Microscopy Studies of Viral Particles Captured Directly From Cell Culture
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چکیده
منابع مشابه
Affinity Cryo-Electron Microscopy Studies of Viral Particles Captured Directly From Cell Culture
Single particle cryo-electron microscopy (cryo-EM) and 3-D reconstruction have emerged in recent years as a powerful tool for solving high-resolution 3-D structures of viruses and macromolecular complexes. However, sample preparation for single-particle cryo-EM typically relies on a dedicated purification step before EM sample grid preparation, for example, using column chromatography or gradie...
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The goal of cryo-electron microscopy (EM) is to reconstruct the 3-dimensional structure of a molecule from a collection of its 2-dimensional projected images. In this paper, we show that the basic premise of cryo-EM—patching together 2-dimensional projections to reconstruct a 3-dimensional object—is naturally one of Čech cohomology with SO(2)-coefficients. We deduce that every cryoEM reconstruc...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2015
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927615003530